Reconstitution & Dosing Calculator

To reconstitute a lyophilized peptide, first wipe the vial stopper and bacteriostatic water vial with an alcohol swab. Draw your chosen volume of bacteriostatic water into an insulin syringe, then inject it slowly into the peptide vial by aiming the stream along the glass wall rather than directly onto the powder. Gently swirl the vial until the powder fully dissolves -- never shake vigorously, as this can denature the peptide. Once reconstituted, store the vial refrigerated at 2-8 degrees Celsius and use within 28-30 days.

The volume of bacteriostatic water depends on the peptide amount in the vial and your desired concentration. A common approach is to add 1 mL (100 units) of bacteriostatic water to a 5 mg vial, creating a concentration of 5 mg/mL (or 5,000 mcg/mL). This makes dose calculation straightforward: each 10 units on an insulin syringe equals 500 mcg. You can adjust the water volume to make doses fall on convenient syringe markings for your specific protocol.

The ideal reconstitution concentration depends on your dose size and syringe type. For most peptides, concentrations between 2-5 mg/mL work well with standard insulin syringes. Higher concentrations (less water) mean smaller injection volumes, which can reduce injection site discomfort. Lower concentrations (more water) provide more precise dosing on syringe graduations. The key is choosing a concentration where your target dose falls on an easy-to-read syringe marking, minimizing measurement error.