Research & Educational Use Only. This protocol guide is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional before beginning any research protocol.
Triple Hormone Receptor Agonist
Weekly Protocol Guide
Retatrutide Protocol Guide
Retatrutide is a triple-action receptor agonist targeting GLP-1, GIP, and glucagon receptors simultaneously, representing next-generation incretin-based research.
Protocol Overview
- Compound
- Retatrutide (GLP-1 / GIP / Glucagon Receptor Agonist)
- Category
- Triple Hormone Receptor Agonist
- Vial Size
- 5mg (5,000mcg)
- Reconstitution
- 2mL bacteriostatic water (2.5mg/mL)
- Route
- Subcutaneous injection
- Frequency
- Once weekly
Reconstitution Instructions
Using 2mL of bacteriostatic water results in a concentration of 2.5mg/mL. Start low and titrate slowly. Always use sterile techniques.
Materials Needed
- 5mg Retatrutide 5mg vial (lyophilized powder)
- 2mL bacteriostatic water (sterile, preserved with 0.9% benzyl alcohol)
- Sterile syringe and needle (18-22 gauge for drawing)
- Alcohol swabs
- Clean workspace
Step-by-Step Process
- Preparation: Allow the Retatrutide 5mg vial and bacteriostatic water to reach room temperature. Wipe the rubber stoppers of both vials with an alcohol swab.
- Draw the Water: Using a sterile syringe, draw up exactly 2mL of bacteriostatic water. Expel any air bubbles.
- Inject into Vial: Insert the needle through the rubber stopper at a slight angle, aiming toward the glass wall. Slowly inject the water down the side of the vial to avoid foaming.
- Mix Gently: Remove the needle. Gently swirl or roll the vial between your hands. Do not shake vigorously.
- Check for Clarity: The solution should be clear once fully reconstituted. Do not use if cloudy.
Titration Dosing Schedule
| Phase | Weeks | Dose | Frequency |
|---|---|---|---|
| Starting | Weeks 1-4 | 0.5mg | Once weekly |
| Titration 1 | Weeks 5-8 | 1mg | Once weekly |
| Titration 2 | Weeks 9-12 | 2mg | Once weekly |
| Titration 3 | Weeks 13-16 | 4mg | Once weekly |
| Maintenance | Weeks 17-24 | 8mg (up to 12mg) | Once weekly |
Administer on the same day each week. Titrate gradually based on tolerability. Do not skip titration steps.
Quick Dosing Reference
| Units (Ticks) | Dose (mcg) | Dose (mg) |
|---|---|---|
| 20 | 500 | 0.5mg |
| 40 | 1000 | 1mg |
| 80 | 2000 | 2mg |
| 160 | 4000 | 4mg |
| 320 | 8000 | 8mg |
| 480 | 12000 | 12mg |
Administration Guidelines
- Administer subcutaneously once weekly on the same day each week
- Rotate injection sites between abdomen, thigh, and upper arm
- Start at lowest dose and titrate very gradually
- Store reconstituted peptide refrigerated at 36-46F (2-8C)
- Use reconstituted solution within 30 days
- Never freeze reconstituted peptides
- Document administration times and observations
Storage Requirements
| State | Storage Conditions |
|---|---|
| Lyophilized (powder) | Room temperature or refrigerated, protect from light |
| Reconstituted | Refrigerated 36-46F (2-8C), use within 30 days |