Dihexa Protocol Guide
Dihexa (N-hexanoyl-Tyr-Ile-(6)-aminohexanoic amide) is a synthetic hexapeptide derived from angiotensin IV and studied as a potent hepatocyte growth factor (HGF) potentiator — reported to be approximately one million times more potent than BDNF in promoting synaptic connectivity. It is administered orally (capsule/powder) or transdermally (1–5% cream), NOT by subcutaneous injection. Due to its extreme potency and long duration of action, it is dosed 1–3x per week rather than daily.
Protocol Overview
- Compound
- Dihexa (N-hexanoyl-Tyr-Ile-(6)-aminohexanoic amide)
- Category
- Nootropic Research Peptide / HGF Potentiator
- Vial Size
- 10mg powder (oral/transdermal use)
- Reconstitution
- Oral: none required (capsule or powder). Transdermal: compound into 1–5% cream base.
- Route
- Oral (capsule/powder) or transdermal cream — NOT subcutaneous injection
- Frequency
- 1–3x per week (long duration of action — do not dose daily)
Preparation Instructions
Dihexa is used orally or transdermally — no injection or reconstitution required. It is extremely potent; always start at the lowest dose and titrate carefully.
Oral (Capsule/Powder)
- 10mg Dihexa powder (lyophilized)
- Capsule machine or pre-filled capsules (if capping manually)
- Milligram-accurate scale for powder measurement
- No solvents or reconstitution needed
- Take on an empty stomach in the morning
Transdermal (1–5% Cream)
- Obtain or compound Dihexa into a lipophilic cream base (DMSO-based or penetration enhancer base).
- Typical concentration: 1–5% Dihexa by weight in cream (10–50mg/g).
- Apply a small amount (pea-sized) to thin-skinned area (inner wrist, inner forearm).
- Rub in thoroughly and allow to absorb before covering.
- Use 1–3x per week only — do not apply daily due to long duration of action.
Dosing Protocol
Dihexa is dosed 1–3x per week, NOT daily. Its extremely long duration of action means daily dosing risks accumulation and overstimulation. Take on an empty stomach in the morning.
| Route | Dose Range | Frequency | Cycle |
|---|---|---|---|
| Oral (capsule/powder) | 10–100mg per dose | 1–3x per week | 4–8 weeks on, 4 weeks off |
| Transdermal (1–5% cream) | Applied topically daily | Once daily (cream route only) | 4–8 weeks on, 4 weeks off |
Start dose: 10–20mg oral, 1x/week. Titrate up to 50–100mg as tolerated. Do NOT use daily oral dosing — long half-life causes accumulation.
Quick Dosing Reference (Oral)
| Dose | Level | Frequency | Notes |
|---|---|---|---|
| 10mg | Starter / Low | 1x/week | Recommended starting point |
| 25mg | Low-Moderate | 1–2x/week | Titrate to after 2–4 weeks at 10mg |
| 50mg | Moderate | 1–2x/week | Common research dose |
| 100mg | High | 1–3x/week | Upper range; experienced researchers only |
Administration Guidelines
- Oral: take in the morning on an empty stomach — use an accurate milligram scale
- Transdermal: apply cream to thin-skinned area (inner wrist/forearm); rub in completely
- Do NOT administer subcutaneously — Dihexa is not a SubQ peptide
- Start with the lowest dose (10mg oral) and do not exceed 1x/week until response is assessed
- Never dose daily orally — Dihexa has a long duration of action that leads to accumulation
- Cycle 4–8 weeks on, 4 weeks off
- Store powder at room temperature or refrigerated, protected from light and moisture
- Compatible stacks: Semax, Selank, Cerebrolysin, NAD+, BPC-157
Storage Requirements
| State | Storage Conditions |
|---|---|
| Powder (sealed) | Room temperature or refrigerated, protect from light and moisture |
| Transdermal cream (compounded) | Refrigerated 36–46°F (2–8°C), use within 60 days |
Stacking Recommendations
Compatible Compounds
- Semax: Complementary BDNF-pathway synergy
- Selank: Anxiolytic support; reduces overstimulation risk
- Cerebrolysin: Neurotrophic factor support via IM protocol
- NAD+: Cellular energy for neuronal metabolism
- BPC-157: Systemic neuroprotection and repair
Popular Stacks
- Cognitive Synergy: Dihexa 25mg oral 2x/week + Semax 600mcg intranasal/day
- Neurotrophic Stack: Dihexa 50mg oral 1x/week + Cerebrolysin 5mL IM/day (cycle)
- Neuroprotective: Dihexa 25mg oral + Selank intranasal + NAD+