Research & Educational Use Only. This protocol guide is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional before beginning any research protocol.
GLP-1 Receptor Agonist
Weekly Protocol Guide
Dulaglutide Protocol Guide
Dulaglutide is a long-acting GLP-1 receptor agonist fused to a modified IgG4 Fc fragment, providing an extended half-life for once-weekly dosing. It is studied for glycemic control and weight management in type 2 diabetes research.
Protocol Overview
- Compound
- Dulaglutide (Trulicity - Weekly GLP-1 for Weight and T2D Research)
- Category
- GLP-1 Receptor Agonist
- Vial Size
- 3mg (3,000mcg)
- Reconstitution
- 2mL bacteriostatic water (1.5mg/mL)
- Route
- Subcutaneous injection
- Frequency
- Once weekly
Reconstitution Instructions
Using 2mL of bacteriostatic water results in a concentration of 1.5mg/mL. Start at 0.75mg and titrate. Always use sterile techniques.
Materials Needed
- 3mg Dulaglutide 3mg vial (lyophilized powder)
- 2mL bacteriostatic water (sterile, preserved with 0.9% benzyl alcohol)
- Sterile syringe and needle (18-22 gauge for drawing)
- Alcohol swabs
- Clean workspace
Step-by-Step Process
- Preparation: Allow the Dulaglutide 3mg vial and bacteriostatic water to reach room temperature. Wipe the rubber stoppers of both vials with an alcohol swab.
- Draw the Water: Using a sterile syringe, draw up exactly 2mL of bacteriostatic water. Expel any air bubbles.
- Inject into Vial: Insert the needle through the rubber stopper at a slight angle, aiming toward the glass wall. Slowly inject the water down the side of the vial to avoid foaming.
- Mix Gently: Remove the needle. Gently swirl or roll the vial between your hands. Do not shake vigorously.
- Check for Clarity: The solution should be clear once fully reconstituted. Do not use if cloudy.
Weekly Dosing Schedule
| Day | Units | Dose | Timing |
|---|---|---|---|
| Monday | 50 | 0.75mg | Morning |
Weekly Total: 50 units (0.75mg) • Vial Duration: ~4 weeks at starting dose
Quick Dosing Reference
| Units (Ticks) | Dose (mcg) | Dose (mg) |
|---|---|---|
| 16.7 | 250 | 0.25mg |
| 33 | 500 | 0.5mg |
| 50 | 750 | 0.75mg |
| 67 | 1000 | 1.0mg |
| 100 | 1500 | 1.5mg |
Administration Guidelines
- Administer subcutaneously once weekly on the same day each week
- Start at 0.75mg weekly for at least 4 weeks then may increase to 1.5mg weekly
- Rotate injection sites between abdomen, thigh, and upper arm
- Store reconstituted peptide refrigerated at 36-46F (2-8C)
- Use reconstituted solution within 30 days
- Never freeze reconstituted peptides
- Document administration times and observations
Storage Requirements
| State | Storage Conditions |
|---|---|
| Lyophilized (powder) | Room temperature or refrigerated, protect from light |
| Reconstituted | Refrigerated 36-46F (2-8C), use within 30 days |