Research & Educational Use Only. This protocol guide is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional before beginning any research protocol.
Telomere Extension Peptide
Weekly Protocol Guide
Epitalon Protocol Guide
Epitalon (Epithalon) is a synthetic tetrapeptide studied for its effects on telomerase activation and telomere elongation. Research suggests it may regulate melatonin production and support cellular longevity through telomere maintenance. Typically administered in 10-20 day cycles.
Protocol Overview
- Compound
- Epitalon (Tetrapeptide Bioregulator (Ala-Glu-Asp-Gly))
- Category
- Telomere Extension Peptide
- Vial Size
- 10mg (10,000mcg)
- Reconstitution
- 2mL bacteriostatic water (5mg/mL)
- Route
- Subcutaneous injection
- Frequency
- 7 days/week
Reconstitution Instructions
Using 2mL of bacteriostatic water results in a concentration of 5mg/mL (5000mcg/mL). Administer in 10-20 day cycles. Always use sterile techniques.
Materials Needed
- 10mg Epitalon 10mg vial (lyophilized powder)
- 2mL bacteriostatic water (sterile, preserved with 0.9% benzyl alcohol)
- Sterile syringe and needle (18-22 gauge for drawing)
- Alcohol swabs
- Clean workspace
Step-by-Step Process
- Preparation: Allow the Epitalon 10mg vial and bacteriostatic water to reach room temperature. Wipe the rubber stoppers of both vials with an alcohol swab.
- Draw the Water: Using a sterile syringe, draw up exactly 2mL of bacteriostatic water. Expel any air bubbles.
- Inject into Vial: Insert the needle through the rubber stopper at a slight angle, aiming toward the glass wall. Slowly inject the water down the side of the vial to avoid foaming.
- Mix Gently: Remove the needle. Gently swirl or roll the vial between your hands. Do not shake vigorously.
- Check for Clarity: The solution should be clear once fully reconstituted. Do not use if cloudy.
Weekly Dosing Schedule
| Day | Units | Dose | Timing |
|---|---|---|---|
| Monday | 50+50 | 2500+2500mcg | AM + PM |
| Tuesday | 50+50 | 2500+2500mcg | AM + PM |
| Wednesday | 50+50 | 2500+2500mcg | AM + PM |
| Thursday | 50+50 | 2500+2500mcg | AM + PM |
| Friday | 50+50 | 2500+2500mcg | AM + PM |
| Saturday | 50+50 | 2500+2500mcg | AM + PM |
| Sunday | 50+50 | 2500+2500mcg | AM + PM |
Weekly Total: 700 units (35,000mcg / 35mg) • Vial Duration: ~2 days at 5mg/day
Quick Dosing Reference
| Units (Ticks) | Dose (mcg) | Dose (mg) |
|---|---|---|
| 10 | 500 | 0.5mg |
| 20 | 1000 | 1mg |
| 50 | 2500 | 2.5mg |
| 100 | 5000 | 5mg |
Administration Guidelines
- Administer subcutaneously, splitting daily dose between AM and PM
- Run 10-20 day cycles with 4-6 month breaks between cycles
- Rotate injection sites between abdomen, thigh, and upper arm
- Store reconstituted peptide refrigerated at 36-46F (2-8C)
- Use reconstituted solution within 30 days
- Never freeze reconstituted peptides
- Document administration times and observations
Storage Requirements
| State | Storage Conditions |
|---|---|
| Lyophilized (powder) | Room temperature or refrigerated, protect from light |
| Reconstituted | Refrigerated 36-46F (2-8C), use within 30 days |