FOXO4-DRI Protocol Guide
FOXO4-DRI is a D-retro-inverso peptide that disrupts the FOXO4-p53 interaction in senescent cells, studied for senolytic (senescent cell clearance) properties. Research suggests it may selectively induce apoptosis in senescent cells while sparing healthy cells, with applications in aging and longevity research.
Protocol Overview
- Compound
- FOXO4-DRI (D-Retro-Inverso FOXO4 Peptide)
- Category
- Senolytic Research Peptide
- Vial Size
- 10mg (10,000mcg)
- Reconstitution
- 2mL bacteriostatic water (5mg/mL)
- Route
- Subcutaneous injection
- Frequency
- 3 consecutive days per quarter (mg/kg dosing)
Reconstitution Instructions
Using 2mL of bacteriostatic water results in a concentration of 5mg/mL (5000mcg/mL). Always use sterile techniques.
Materials Needed
- 10mg FOXO4-DRI 10mg vial (lyophilized powder)
- 2mL bacteriostatic water (sterile, preserved with 0.9% benzyl alcohol)
- Sterile syringe and needle (18-22 gauge for drawing)
- Alcohol swabs
- Clean workspace
Step-by-Step Process
- Preparation: Allow the FOXO4-DRI 10mg vial and bacteriostatic water to reach room temperature. Wipe the rubber stoppers of both vials with an alcohol swab.
- Draw the Water: Using a sterile syringe, draw up exactly 2mL of bacteriostatic water. Expel any air bubbles.
- Inject into Vial: Insert the needle through the rubber stopper at a slight angle, aiming toward the glass wall. Slowly inject the water down the side of the vial to avoid foaming.
- Mix Gently: Remove the needle. Gently swirl or roll the vial between your hands. Do not shake vigorously.
- Check for Clarity: The solution should be clear once fully reconstituted. Do not use if cloudy.
Quarterly Dosing Protocol (mg/kg)
FOXO4-DRI is a senolytic peptide researched using a pulsed, quarterly approach — 3 consecutive days per cycle, dosed by body weight. This mirrors the dosing used in preclinical literature (van Deursen / Baker lab protocols).
| Body Weight | Dose (3 mg/kg) | Dose (5 mg/kg) | Volume at 5mg/mL |
|---|---|---|---|
| 60 kg (132 lbs) | 180 mg | 300 mg | 36–60 mL |
| 70 kg (154 lbs) | 210 mg | 350 mg | 42–70 mL |
| 80 kg (176 lbs) | 240 mg | 400 mg | 48–80 mL |
| 90 kg (198 lbs) | 270 mg | 450 mg | 54–90 mL |
| 100 kg (220 lbs) | 300 mg | 500 mg | 60–100 mL |
Cycle: 3 days on, ~87 days off • Frequency: Once per quarter • Timing: Morning subcutaneous injection
Reconstitution for mg/kg Dosing
Because doses are in the hundreds of mg range, FOXO4-DRI must be reconstituted at higher concentrations using multiple vials. Each 10mg vial reconstituted in 2mL = 5mg/mL concentration.
| Vials Needed (10mg each) | Total Peptide | BAC Water Required | Approximate Body Weight Coverage |
|---|---|---|---|
| 20 vials | 200 mg | 40 mL | ~67 kg at 3 mg/kg |
| 25 vials | 250 mg | 50 mL | ~83 kg at 3 mg/kg |
| 35 vials | 350 mg | 70 mL | ~70 kg at 5 mg/kg |
| 50 vials | 500 mg | 100 mL | ~100 kg at 5 mg/kg |
Note: FOXO4-DRI is one of the most expensive research peptides per cycle. Costs at 3–5 mg/kg can range from several hundred to several thousand dollars per quarterly cycle.
Administration Guidelines
- Administer subcutaneously on 3 consecutive days per quarter (quarterly cycle)
- Dose is calculated by body weight: 3–5 mg/kg per injection, per day × 3 days
- Rotate injection sites between abdomen, thigh, and upper arm
- Store reconstituted peptide refrigerated at 36-46F (2-8C)
- Use reconstituted solution within 30 days
- Never freeze reconstituted peptides
- Document administration times and observations
Storage Requirements
| State | Storage Conditions |
|---|---|
| Lyophilized (powder) | Room temperature or refrigerated, protect from light |
| Reconstituted | Refrigerated 36-46F (2-8C), use within 30 days |