Research & Educational Use Only. This protocol guide is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional before beginning any research protocol.
Research Compound
Weekly Protocol Guide
Glutathione Protocol Guide
Glutathione is a tripeptide and the most abundant intracellular antioxidant, playing a crucial role in detoxification, immune function, and protection against oxidative stress.
Protocol Overview
- Compound
- Glutathione (L-Glutathione (Reduced))
- Category
- Research Compound
- Vial Size
- 200mg (200,000mcg)
- Reconstitution
- 2mL bacteriostatic water (100mg/mL)
- Route
- Subcutaneous injection
- Frequency
- 3 days/week
Reconstitution Instructions
Using 2mL of bacteriostatic water results in a concentration of 100mg/mL. Glutathione dissolves readily. Always use sterile techniques.
Materials Needed
- 200mg Glutathione vial (lyophilized powder)
- 2mL bacteriostatic water (sterile, preserved with 0.9% benzyl alcohol)
- Sterile syringe and needle (18-22 gauge for drawing)
- Alcohol swabs
- Clean workspace
Step-by-Step Process
- Preparation: Allow the Glutathione vial and bacteriostatic water to reach room temperature. Wipe the rubber stoppers of both vials with an alcohol swab.
- Draw the Water: Using a sterile syringe, draw up exactly 2mL of bacteriostatic water. Expel any air bubbles.
- Inject into Vial: Insert the needle through the rubber stopper at a slight angle, aiming toward the glass wall. Slowly inject the water down the side of the vial to avoid foaming.
- Mix Gently: Remove the needle. Gently swirl or roll the vial between your hands. Do not shake vigorously.
- Check for Clarity: The solution should be clear once fully reconstituted. Do not use if cloudy.
Weekly Dosing Schedule
| Day | Units | Dose | Timing |
|---|---|---|---|
| Monday | 50 | 50mg | Morning |
| Wednesday | 5 | 50mg | Morning |
| Friday | 50 | 50mg | Morning |
Weekly Total: 150 units (150mg) • Vial Duration: ~4 doses (~1.3 weeks)
Quick Dosing Reference
| Units (Ticks) | Dose (mcg) | Dose (mg) |
|---|---|---|
| 2 | 20mg | 0.02g |
| 5 | 50mg | 0.05g |
| 10 | 100mg | 0.1g |
| 20 | 200mg | 0.2g |
Administration Guidelines
- Administer subcutaneously or intramuscularly in the morning
- Rotate injection sites between abdomen, thigh, and upper arm
- Store reconstituted solution refrigerated at 36-46F (2-8C)
- Use reconstituted solution within 30 days
- Never freeze reconstituted solution
- Document administration times and observations
Storage Requirements
| State | Storage Conditions |
|---|---|
| Lyophilized (powder) | Room temperature or refrigerated, protect from light |
| Reconstituted | Refrigerated 36-46F (2-8C), use within 30 days |