Research & Educational Use Only. This protocol guide is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional before beginning any research protocol.
GnRH Analog for HPTA Maintenance
Weekly Protocol Guide
Gonadorelin Protocol Guide
Gonadorelin is a synthetic analog of endogenous GnRH (gonadotropin-releasing hormone). It is used in research to maintain hypothalamic-pituitary-gonadal axis (HPTA) function, stimulating pulsatile LH and FSH release to preserve natural hormone signaling during exogenous hormone protocols.
Protocol Overview
- Compound
- Gonadorelin (GnRH — Gonadotropin-Releasing Hormone)
- Category
- Reproductive Health Peptide / PCT Support
- Chemical Name
- pyro-Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2
- Vial Size
- 10mg vial
- Reconstitution
- 10mL bacteriostatic water → 1mg/mL (1,000mcg/mL)
- Route
- Subcutaneous injection
- Frequency
- 2–3x per week (protocol dependent)
Reconstitution Instructions
Add 10mL of bacteriostatic water to a 10mg Gonadorelin vial for a concentration of 1mg/mL (1,000mcg/mL). Always use sterile technique.
Materials Needed
- 10mg Gonadorelin vial (lyophilized powder)
- 10mL bacteriostatic water (sterile, preserved with 0.9% benzyl alcohol)
- 3–5mL sterile syringe and needle (18–22 gauge for drawing)
- Insulin syringe (29–31 gauge) for SubQ injection
- Alcohol swabs
- Clean workspace
Step-by-Step Process
- Allow the Gonadorelin vial and bacteriostatic water to reach room temperature. Wipe rubber stoppers with an alcohol swab.
- Draw up exactly 10mL of bacteriostatic water using a sterile syringe. Expel air bubbles.
- Insert the needle through the rubber stopper at a slight angle and slowly inject the water down the side of the vial to avoid foaming.
- Remove the needle. Gently swirl or roll the vial between your hands. Do not shake vigorously.
- The solution should be clear. Final concentration: 1mg/mL (1,000mcg/mL). Store refrigerated.
Dosing Protocol
Gonadorelin dose depends on the research application. For TRT support, 250mcg twice weekly is the most common protocol. For fertility/PCT, 500mcg 3x/week is used.
| Protocol | Dose | Frequency | Route | Cycle |
|---|---|---|---|---|
| TRT Support (LH/FSH maintenance) | 250mcg | 2x/week (e.g., Mon/Thu) | SubQ | Ongoing with TRT |
| Conservative / Starter | 100mcg | 2–3x/week | SubQ | Ongoing or 8–12 weeks |
| Fertility Research | 500mcg | 3x/week | SubQ | 8–12 weeks |
| PCT Support | 250–500mcg | 2–3x/week | SubQ | 8–12 weeks |
Standard TRT Support: 250mcg SubQ twice weekly (Mon/Thu) alongside exogenous hormone protocol. Vial lasts ~20 weeks at this dose.
Quick Dosing Reference (at 1mg/mL — 10mg vial + 10mL BAC water)
| Units (Insulin Syringe) | Dose (mcg) | Dose (mg) | Protocol Use |
|---|---|---|---|
| 10 | 100mcg | 0.1mg | Conservative starter |
| 25 | 250mcg | 0.25mg | Standard TRT support |
| 50 | 500mcg | 0.5mg | Fertility / PCT protocol |
Administration Guidelines
- Administer subcutaneously in the lower abdomen or thigh using a 29–31 gauge insulin syringe
- Rotate injection sites with each administration
- Standard TRT support protocol: 250mcg SubQ twice weekly (Monday/Thursday)
- For fertility/PCT: 500mcg SubQ 3x/week
- Timing alongside TRT: administer on the same days as testosterone injection or opposite days
- Store reconstituted peptide refrigerated at 36–46°F (2–8°C)
- Use reconstituted solution within 30 days
- Never freeze reconstituted peptides
- Cycle: ongoing alongside TRT, or 8–12 weeks for PCT purposes
Storage Requirements
| State | Storage Conditions |
|---|---|
| Lyophilized (powder) | Room temperature or refrigerated, protect from light |
| Reconstituted (1mg/mL) | Refrigerated 36–46°F (2–8°C), use within 30 days |
Stacking Recommendations
Compatible Compounds
- HCG: Synergistic LH mimetic for testicular volume maintenance
- Enclomiphene: Selective estrogen receptor modulator for FSH/LH support
- Kisspeptin-10: Upstream GnRH stimulator to enhance pulsatile release
Popular Stacks
- TRT Maintenance: Gonadorelin 250mcg SubQ 2x/week + HCG 500–1000 IU 2x/week
- PCT Protocol: Gonadorelin 500mcg 3x/week + Enclomiphene 12.5–25mg/day
- Fertility Research: Gonadorelin 500mcg 3x/week + Kisspeptin-10 200mcg 2x/week