Research & Educational Use Only. This protocol guide is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional before beginning any research protocol.
Fertility Preservation / TRT Adjunct
Weekly Protocol Guide
HCG 5000IU Protocol Guide
✓ Verified dosing (fact-checked against FDA labels / published trials): Route: the FDA label specifies intramuscular, but subcutaneous is widely used and accepted in fertility/TRT research practice (off-label, effective). Male hypogonadotropic hypogonadism: 500-1,000 USP Units IM 3x/week x3 wks, then 2x/week x3 wks; OR 4,000 USP Units IM 3x/week x6-9 mo, then 2,000 USP Units 3x/week x3 mo. Doses are in USP Units, reconstituted from 5,000 or 10,000-unit vials. The "2-3x per week" frequency is consistent with the male regimen, and the route is flexible — IM per label, or SubQ in common practice. This supersedes any conflicting figures shown elsewhere on this page; confirm with a licensed provider.
HCG is a naturally occurring hormone used in research for fertility preservation and as a TRT adjunct. It mimics luteinizing hormone (LH) to stimulate testicular function, supporting endogenous testosterone production and spermatogenesis during exogenous hormone protocols.
Protocol Overview
- Compound
- HCG 5000IU (Human Chorionic Gonadotropin)
- Category
- Fertility Preservation / TRT Adjunct
- Vial Size
- 5000IU (5,000 IU)
- Reconstitution
- 2mL bacteriostatic water (2,500 IU/mL)
- Route
- Subcutaneous injection
- Frequency
- 3 days/week
Reconstitution Instructions
Using 2mL of bacteriostatic water results in a concentration of 2,500 IU/mL. Always use sterile techniques.
Materials Needed
- 5000IU HCG 5000IU vial (lyophilized powder)
- 2mL bacteriostatic water (sterile, preserved with 0.9% benzyl alcohol)
- Sterile syringe and needle (18-22 gauge for drawing)
- Alcohol swabs
- Clean workspace
Step-by-Step Process
- Preparation: Allow the HCG 5000IU vial and bacteriostatic water to reach room temperature. Wipe the rubber stoppers of both vials with an alcohol swab.
- Draw the Water: Using a sterile syringe, draw up exactly 2mL of bacteriostatic water. Expel any air bubbles.
- Inject into Vial: Insert the needle through the rubber stopper at a slight angle, aiming toward the glass wall. Slowly inject the water down the side of the vial to avoid foaming.
- Mix Gently: Remove the needle. Gently swirl or roll the vial between your hands. Do not shake vigorously.
- Check for Clarity: The solution should be clear once fully reconstituted. Do not use if cloudy.
Administration Guidelines
- Administer subcutaneously or intramuscularly as directed
- Rotate injection sites between abdomen, thigh, and upper arm
- Common protocol is 250-500IU 2-3x per week alongside TRT
- Store reconstituted peptide refrigerated at 36-46F (2-8C)
- Use reconstituted solution within 30 days
- Never freeze reconstituted peptides
- Document administration times and observations
Storage Requirements
| State | Storage Conditions |
|---|---|
| Lyophilized (powder) | Room temperature or refrigerated, protect from light |
| Reconstituted | Refrigerated 36-46F (2-8C), use within 30 days |