Research & Educational Use Only. This protocol guide is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional before beginning any research protocol.
Mitochondrial Research Peptide
Weekly Protocol Guide
MOTS-C Protocol Guide
MOTS-C is a mitochondrial-derived peptide studied for metabolic regulation and energy homeostasis. This larger vial supports extended research protocols.
Protocol Overview
- Compound
- MOTS-C (Mitochondrial Open Reading Frame of the 12S rRNA Type-C)
- Category
- Mitochondrial Research Peptide
- Vial Size
- 20mg (20,000mcg)
- Reconstitution
- 2mL bacteriostatic water (10mg/mL)
- Route
- Subcutaneous injection
- Frequency
- 1–3 days/week (5–10mg total per week)
Reconstitution Instructions
Using 2mL results in a concentration of 10mg/mL. Always use sterile techniques.
Materials Needed
- 20mg MOTS-C 20mg vial (lyophilized powder)
- 2mL bacteriostatic water (sterile, preserved with 0.9% benzyl alcohol)
- Sterile syringe and needle (18-22 gauge for drawing)
- Alcohol swabs
- Clean workspace
Step-by-Step Process
- Preparation: Allow the MOTS-C 20mg vial and bacteriostatic water to reach room temperature. Wipe the rubber stoppers of both vials with an alcohol swab.
- Draw the Water: Using a sterile syringe, draw up exactly 2mL of bacteriostatic water. Expel any air bubbles.
- Inject into Vial: Insert the needle through the rubber stopper at a slight angle, aiming toward the glass wall. Slowly inject the water down the side of the vial to avoid foaming.
- Mix Gently: Remove the needle. Gently swirl or roll the vial between your hands. Do not shake vigorously.
- Check for Clarity: The solution should be clear once fully reconstituted. Do not use if cloudy.
Weekly Dosing Schedule
MOTS-C is typically administered at 5–10mg per week, either as a single weekly injection or split across 2–3 doses.
| Protocol | Schedule | Dose Per Injection | Weekly Total |
|---|---|---|---|
| Standard | Once weekly | 5mg (50 units) | 5mg |
| Split dose | Mon / Thu | 2.5mg (25 units) each | 5mg |
| Higher dose | Mon / Wed / Fri | 3.3mg (~33 units) each | ~10mg |
Standard dose: 5mg/week • Vial Duration: ~4 weeks at 5mg/week (20mg vial) • Administer in the morning on an empty stomach
Quick Dosing Reference (at 10mg/mL)
| Units (Ticks) | Dose (mcg) | Dose (mg) |
|---|---|---|
| 10 | 1,000 | 1mg |
| 25 | 2,500 | 2.5mg |
| 33 | 3,300 | 3.3mg |
| 50 | 5,000 | 5mg |
| 100 | 10,000 | 10mg |
Administration Guidelines
- Administer subcutaneously in the morning on an empty stomach
- Rotate injection sites between abdomen, thigh, and upper arm
- Store reconstituted peptide refrigerated at 36-46F (2-8C)
- Use reconstituted solution within 30 days
- Never freeze reconstituted peptides
- Document administration times and observations
Storage Requirements
| State | Storage Conditions |
|---|---|
| Lyophilized (powder) | Room temperature or refrigerated, protect from light |
| Reconstituted | Refrigerated 36-46F (2-8C), use within 30 days |