Research & Educational Use Only. This protocol guide is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional before beginning any research protocol.
Research Compound
Weekly Protocol Guide
NAD+ Protocol Guide
NAD+ is a coenzyme found in all living cells, critical for cellular energy metabolism, DNA repair, and cell signaling. Research focuses on aging, mitochondrial function, and neuroprotection.
Protocol Overview
- Compound
- NAD+ (Nicotinamide Adenine Dinucleotide)
- Category
- Research Compound
- Vial Size
- 1000mg (1,000,000mcg)
- Reconstitution
- 10mL bacteriostatic water (100mg/mL)
- Route
- Subcutaneous injection (or IV for clinical)
- Frequency
- Daily or 3-5x/week
Reconstitution Instructions
Using 10mL of bacteriostatic water results in a concentration of 100mg/mL. NAD+ dissolves readily. Always use sterile techniques.
Materials Needed
- 1000mg NAD+ vial (lyophilized powder)
- 10mL bacteriostatic water (sterile, preserved with 0.9% benzyl alcohol)
- Sterile syringe and needle (18-22 gauge for drawing)
- Alcohol swabs
- Clean workspace
Step-by-Step Process
- Preparation: Allow the NAD+ vial and bacteriostatic water to reach room temperature. Wipe the rubber stoppers of both vials with an alcohol swab.
- Draw the Water: Using a sterile syringe, draw up exactly 10mL of bacteriostatic water. Expel any air bubbles.
- Inject into Vial: Insert the needle through the rubber stopper at a slight angle, aiming toward the glass wall. Slowly inject the water down the side of the vial to avoid foaming.
- Mix Gently: Remove the needle. Gently swirl or roll the vial between your hands. Do not shake vigorously.
- Check for Clarity: The solution should be clear once fully reconstituted. Do not use if cloudy.
Dosing Schedule
| Protocol | Dose | Frequency | Route |
|---|---|---|---|
| SubQ Standard | 100-250mg | Daily or 3-5x/week | Subcutaneous |
| SubQ Loading | 500mg daily x 5 days, then 250mg 3x/week | See description | Subcutaneous |
| IV Clinical | 250-750mg per infusion | 1-2x/week | Intravenous (clinical only) |
Weekly Total (SubQ standard): 500-1,750mg • Vial Duration: ~1-2 weeks at standard dosing
Quick Dosing Reference
| Volume (100mg/mL) | Dose | Notes |
|---|---|---|
| 0.5mL | 50mg | Low / starter |
| 1.0mL | 100mg | Standard low |
| 2.5mL | 250mg | Standard |
| 5.0mL | 500mg | Loading dose |
Administration Guidelines
- Administer subcutaneously in the morning
- Start with lower doses as NAD+ can cause flushing
- Rotate injection sites between abdomen, thigh, and upper arm
- Store reconstituted solution refrigerated at 36-46F (2-8C)
- Use reconstituted solution within 30 days
- Never freeze reconstituted solution
- Document administration times and observations
Storage Requirements
| State | Storage Conditions |
|---|---|
| Lyophilized (powder) | Room temperature or refrigerated, protect from light |
| Reconstituted | Refrigerated 36-46F (2-8C), use within 30 days |