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Research & Educational Use Only. This protocol guide is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional before beginning any research protocol.
Nootropic Research Peptide Weekly Protocol Guide

Semax Protocol Guide

✓ Verified dosing (fact-checked against FDA labels / published trials): Registered route is intranasal, but subcutaneous injection is also commonly used in research practice. Registered (Russia) 0.1% solution for cognition/fatigue: 400-900 mcg TOTAL per day, DIVIDED into 2-3 intranasal administrations, 3-14 day courses (pediatric cognitive: 200-400 mcg/day in 2 doses, 30 days). Acute ischemic stroke (1% solution, inpatient): 6,000-20,000 mcg/day divided across 3-6 intranasal doses for ~10… This supersedes any conflicting figures shown elsewhere on this page; confirm with a licensed provider.

Semax is a synthetic peptide derived from ACTH fragment 4-10, studied for cognitive enhancement and neuroprotection.

Protocol Overview

Compound
Semax (Synthetic ACTH(4-10) Analog)
Category
Nootropic Research Peptide
Vial Size
5mg (5,000mcg)
Reconstitution
2mL bacteriostatic water (2.5mg/mL)
Route
Subcutaneous injection
Frequency
5 days/week

Reconstitution Instructions

Using 2mL results in a concentration of 2.5mg/mL. Always use sterile techniques.

Materials Needed

  • 5mg Semax 5mg vial (lyophilized powder)
  • 2mL bacteriostatic water (sterile, preserved with 0.9% benzyl alcohol)
  • Sterile syringe and needle (18-22 gauge for drawing)
  • Alcohol swabs
  • Clean workspace

Step-by-Step Process

  1. Preparation: Allow the Semax 5mg vial and bacteriostatic water to reach room temperature. Wipe the rubber stoppers of both vials with an alcohol swab.
  2. Draw the Water: Using a sterile syringe, draw up exactly 2mL of bacteriostatic water. Expel any air bubbles.
  3. Inject into Vial: Insert the needle through the rubber stopper at a slight angle, aiming toward the glass wall. Slowly inject the water down the side of the vial to avoid foaming.
  4. Mix Gently: Remove the needle. Gently swirl or roll the vial between your hands. Do not shake vigorously.
  5. Check for Clarity: The solution should be clear once fully reconstituted. Do not use if cloudy.

Administration Guidelines

  • Administer intranasally or subcutaneously in the morning
  • If intranasal, apply 1-2 drops per nostril
  • Rotate injection sites if using subcutaneous
  • Store reconstituted peptide refrigerated at 36-46F (2-8C)
  • Use reconstituted solution within 30 days
  • Never freeze reconstituted peptides
  • Document administration times and observations

Storage Requirements

StateStorage Conditions
Lyophilized (powder)Room temperature or refrigerated, protect from light
ReconstitutedRefrigerated 36-46F (2-8C), use within 30 days
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⚠ Before any use: human safety data is limited or absent for many of these compounds. Consult a licensed healthcare provider first — especially if you are pregnant or nursing, have a medical condition, or take other medications.