Research & Educational Use Only. This protocol guide is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional before beginning any research protocol.
Nootropic Research Peptide
Weekly Protocol Guide
Semax Protocol Guide
Semax is a synthetic peptide derived from ACTH fragment 4-10, studied for cognitive enhancement and neuroprotection.
Protocol Overview
- Compound
- Semax (Synthetic ACTH(4-10) Analog)
- Category
- Nootropic Research Peptide
- Vial Size
- 5mg (5,000mcg)
- Reconstitution
- 2mL bacteriostatic water (2.5mg/mL)
- Route
- Subcutaneous injection
- Frequency
- 5 days/week
Reconstitution Instructions
Using 2mL results in a concentration of 2.5mg/mL. Always use sterile techniques.
Materials Needed
- 5mg Semax 5mg vial (lyophilized powder)
- 2mL bacteriostatic water (sterile, preserved with 0.9% benzyl alcohol)
- Sterile syringe and needle (18-22 gauge for drawing)
- Alcohol swabs
- Clean workspace
Step-by-Step Process
- Preparation: Allow the Semax 5mg vial and bacteriostatic water to reach room temperature. Wipe the rubber stoppers of both vials with an alcohol swab.
- Draw the Water: Using a sterile syringe, draw up exactly 2mL of bacteriostatic water. Expel any air bubbles.
- Inject into Vial: Insert the needle through the rubber stopper at a slight angle, aiming toward the glass wall. Slowly inject the water down the side of the vial to avoid foaming.
- Mix Gently: Remove the needle. Gently swirl or roll the vial between your hands. Do not shake vigorously.
- Check for Clarity: The solution should be clear once fully reconstituted. Do not use if cloudy.
Weekly Dosing Schedule
| Day | Units | Dose | Timing |
|---|---|---|---|
| Monday | 12 | 300mcg | Morning |
| Tuesday | 12 | 300mcg | Morning |
| Wednesday | 12 | 300mcg | Morning |
| Thursday | 12 | 300mcg | Morning |
| Friday | 12 | 300mcg | Morning |
Weekly Total: 60 units (1,500mcg) • Vial Duration: ~23 days (~3.3 weeks)
Quick Dosing Reference
| Units (Ticks) | Dose (mcg) | Dose (mg) |
|---|---|---|
| 2 | 50 | 0.05mg |
| 4 | 100 | 0.1mg |
| 8 | 200 | 0.2mg |
| 10 | 250 | 0.25mg |
| 20 | 500 | 0.5mg |
Administration Guidelines
- Administer intranasally or subcutaneously in the morning
- If intranasal, apply 1-2 drops per nostril
- Rotate injection sites if using subcutaneous
- Store reconstituted peptide refrigerated at 36-46F (2-8C)
- Use reconstituted solution within 30 days
- Never freeze reconstituted peptides
- Document administration times and observations
Storage Requirements
| State | Storage Conditions |
|---|---|
| Lyophilized (powder) | Room temperature or refrigerated, protect from light |
| Reconstituted | Refrigerated 36-46F (2-8C), use within 30 days |