Research & Educational Use Only. This protocol guide is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional before beginning any research protocol.
Research Peptide
Weekly Protocol Guide
TB-500 Protocol Guide
TB-500 is a synthetic version of Thymosin Beta-4, a naturally occurring peptide in virtually all human and animal cells. It is studied for tissue repair, cell migration, and inflammation modulation.
Protocol Overview
- Compound
- TB-500 (Thymosin Beta-4 Fragment)
- Category
- Research Peptide
- Vial Size
- 10mg (10,000mcg)
- Reconstitution
- 2mL bacteriostatic water (5mg/mL)
- Route
- Subcutaneous injection
- Frequency
- 2x/week (loading) then 1x/week (maintenance)
Reconstitution Instructions
Using 2mL of bacteriostatic water results in a concentration of 5mg/mL. Always use sterile techniques.
Materials Needed
- 10mg TB-500 vial (lyophilized powder)
- 2mL bacteriostatic water (sterile, preserved with 0.9% benzyl alcohol)
- Sterile syringe and needle (18-22 gauge for drawing)
- Alcohol swabs
- Clean workspace
Step-by-Step Process
- Preparation: Allow the TB-500 vial and bacteriostatic water to reach room temperature. Wipe the rubber stoppers of both vials with an alcohol swab.
- Draw the Water: Using a sterile syringe, draw up exactly 2mL of bacteriostatic water. Expel any air bubbles.
- Inject into Vial: Insert the needle through the rubber stopper at a slight angle, aiming toward the glass wall. Slowly inject the water down the side of the vial to avoid foaming.
- Mix Gently: Remove the needle. Gently swirl or roll the vial between your hands. Do not shake vigorously.
- Check for Clarity: The solution should be clear once fully reconstituted. Do not use if cloudy.
Weekly Dosing Schedule
TB-500 protocols typically have two phases: a loading phase (4–6 weeks) to build tissue levels, followed by a maintenance phase.
| Phase | Schedule | Dose Per Injection | Weekly Total |
|---|---|---|---|
| Loading (Weeks 1–6) | 2x/week (Mon & Thu) | 2.5mg (50 units) | 5mg |
| Maintenance (Weeks 7+) | 1x/week | 2.5mg (50 units) | 2.5mg |
Loading phase: 5mg/week • Maintenance: 2.5mg/week • Vial Duration: 1 week loading or 2 weeks maintenance (10mg vial)
Quick Dosing Reference (at 5mg/mL)
| Units (Ticks) | Dose (mcg) | Dose (mg) |
|---|---|---|
| 10 | 500 | 0.5mg |
| 20 | 1,000 | 1mg |
| 30 | 1,500 | 1.5mg |
| 40 | 2,000 | 2mg |
| 50 | 2,500 | 2.5mg |
Administration Guidelines
- Administer subcutaneously in the morning on an empty stomach
- Rotate injection sites between abdomen, thigh, and upper arm
- Store reconstituted peptide refrigerated at 36-46F (2-8C)
- Use reconstituted solution within 30 days
- Never freeze reconstituted peptides
- Document administration times and observations
Storage Requirements
| State | Storage Conditions |
|---|---|
| Lyophilized (powder) | Room temperature or refrigerated, protect from light |
| Reconstituted | Refrigerated 36-46F (2-8C), use within 30 days |