Research & Educational Use Only. This protocol guide is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional before beginning any research protocol.
Tissue Repair / Recovery Peptide
Weekly Protocol Guide
Thymosin Beta-4 Protocol Guide
Thymosin Beta-4 (TB-4) is the full-length 43-amino acid peptide studied for tissue repair, wound healing, and anti-inflammatory properties. Unlike the truncated fragment TB-500, full-length TB-4 retains all biological activity domains including actin sequestration, anti-inflammatory signaling, and stem cell migration promotion.
Protocol Overview
- Compound
- Thymosin Beta-4 (Full-Length Thymosin Beta-4 (43 Amino Acids))
- Category
- Tissue Repair / Recovery Peptide
- Vial Size
- 5mg (5,000mcg)
- Reconstitution
- 2mL bacteriostatic water (2.5mg/mL)
- Route
- Subcutaneous injection
- Frequency
- 2 days/week
Reconstitution Instructions
Using 2mL of bacteriostatic water results in a concentration of 2.5mg/mL. Always use sterile techniques.
Materials Needed
- 5mg Thymosin Beta-4 5mg vial (lyophilized powder)
- 2mL bacteriostatic water (sterile, preserved with 0.9% benzyl alcohol)
- Sterile syringe and needle (18-22 gauge for drawing)
- Alcohol swabs
- Clean workspace
Step-by-Step Process
- Preparation: Allow the Thymosin Beta-4 5mg vial and bacteriostatic water to reach room temperature. Wipe the rubber stoppers of both vials with an alcohol swab.
- Draw the Water: Using a sterile syringe, draw up exactly 2mL of bacteriostatic water. Expel any air bubbles.
- Inject into Vial: Insert the needle through the rubber stopper at a slight angle, aiming toward the glass wall. Slowly inject the water down the side of the vial to avoid foaming.
- Mix Gently: Remove the needle. Gently swirl or roll the vial between your hands. Do not shake vigorously.
- Check for Clarity: The solution should be clear once fully reconstituted. Do not use if cloudy.
Weekly Dosing Schedule
| Day | Units | Dose | Timing |
|---|---|---|---|
| Monday | 30 | 750mcg | Morning |
| Thursday | 30 | 750mcg | Morning |
Weekly Total: 60 units (1,500mcg) • Vial Duration: ~17 days
Quick Dosing Reference
| Units (Ticks) | Dose (mcg) | Dose (mg) |
|---|---|---|
| 4 | 100 | 0.1mg |
| 10 | 250 | 0.25mg |
| 20 | 500 | 0.5mg |
| 30 | 750 | 0.75mg |
| 40 | 1000 | 1mg |
Administration Guidelines
- Administer subcutaneously twice per week (e.g., Monday and Thursday)
- Rotate injection sites between abdomen, thigh, and upper arm
- Inject near area of injury when possible for localized research
- Store reconstituted peptide refrigerated at 36-46F (2-8C)
- Use reconstituted solution within 30 days
- Never freeze reconstituted peptides
- Document administration times, sites, and observations
Storage Requirements
| State | Storage Conditions |
|---|---|
| Lyophilized (powder) | Room temperature or refrigerated, protect from light |
| Reconstituted | Refrigerated 36-46F (2-8C), use within 30 days |