Reconstitution Quick Guide
Step-by-step · Dosing math · Printable · Updated April 2026
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Step-by-Step Reconstitution
6 Steps-
1
Gather Supplies
You need: lyophilized peptide vial, bacteriostatic water (BAC water), insulin syringe (29-31 gauge), and alcohol swabs. Work on a clean, flat surface.
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2
Calculate Water Volume
Decide how much BAC water to add based on your vial size and desired concentration. See the dosing math table below for common calculations.
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3
Draw BAC Water into Syringe
Swab the BAC water vial top with alcohol. Insert syringe and draw your calculated volume slowly, removing air bubbles.
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4
Inject Slowly Down Side of Vial
Swab the peptide vial top with alcohol. Insert needle and release BAC water slowly down the inside wall of the vial. Never spray directly onto the powder — this can damage peptide bonds.
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5
Gently Swirl to Dissolve
Gently swirl or roll the vial between your palms until the powder fully dissolves. The solution should be clear. Never shake — agitation degrades peptides.
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6
Refrigerate Immediately
Store reconstituted peptide at 2–8°C (standard refrigerator). Use within 28 days. Label the vial with the date and concentration.
Dosing Math — 5mg Vial + 2mL BAC Water
ReferenceConcentration: 2,500 mcg/mL (2.5 mg/mL)
| Draw Volume | Syringe Units | Dose |
|---|---|---|
0.05 mL | 5 units | 125 mcg |
0.1 mL | 10 units | 250 mcg |
0.15 mL | 15 units | 375 mcg |
0.2 mL | 20 units | 500 mcg |
0.4 mL | 40 units | 1,000 mcg (1 mg) |
Common Vial Sizes — Water Volume Guide
Quick Ref| Vial Size | BAC Water | Concentration | 0.1mL Dose |
|---|---|---|---|
| 5 mg | 1 mL | 5,000 mcg/mL | 500 mcg |
| 5 mg | 2 mL | 2,500 mcg/mL | 250 mcg |
| 10 mg | 2 mL | 5,000 mcg/mL | 500 mcg |
| 10 mg | 4 mL | 2,500 mcg/mL | 250 mcg |
| 15 mg | 3 mL | 5,000 mcg/mL | 500 mcg |
| 15 mg | 6 mL | 2,500 mcg/mL | 250 mcg |
Formula: Concentration = (Vial mg × 1000) ÷ Water mL = mcg per mL
Storage Rules
Critical| Temperature | 2 – 8°C (standard refrigerator) |
| Light Exposure | Keep away from direct light. Store in original box or wrap in foil. |
| Shelf Life | 28 days after reconstitution |
| Unreconstituted | Refrigerate or freeze for long-term storage. Room temp is OK short-term. |
| Never | Freeze reconstituted peptide. Leave at room temp. Use cloudy solution. |
Common Mistakes to Avoid
Warning-
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Spraying water directly on powder — This can denature the peptide. Always aim the stream at the vial wall and let it trickle down.
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×
Shaking the vial — Aggressive agitation breaks peptide bonds. Gently swirl or roll instead.
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×
Using sterile water instead of BAC water — Sterile water has no preservative. BAC water contains 0.9% benzyl alcohol to prevent bacterial growth over multiple uses.
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×
Leaving reconstituted vial at room temp — Peptides degrade rapidly outside refrigeration. Return to fridge immediately after each use.
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×
Using past 28 days — Potency and sterility decrease over time. Discard and reconstitute a fresh vial.
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×
Skipping alcohol swabs — Always swab vial tops before inserting needles to prevent contamination.
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✓
Label every vial — Write the peptide name, concentration, and reconstitution date on each vial.